Lisa Ziegert was abducted, raped, and killed in the Town of Agawam, Massachusetts in April 1992. Over the course of the investigation, state, local and federal investigators followed several hundred leads documenting what they learned. While a single source male DNA profile was developed and uploaded to local, state, national and international databases, for over fifteen years, it yielded no hits, and analyses of over 320 additional buccal or blood samples obtained by investigators provided no match to the known profile. This case study will describe the multi-jurisdictional law enforcement investigation that pursued until her assailant’s arrest in September 2017, and conviction for first-degree murder in 2019. The presenters will illustrate how they employed strategic multidisciplinary efforts that proved pivotal in the trajectory of this case, leading to the identification, arrest and prosecution of Lisa’s assailant; a near-fifty-year-old resident of a neighboring town who had no known criminal history.